|
KMID : 1094720110160061187
|
|
Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 6 p.1187 ~ p.1195
|
|
|
Thermo-sensitive amphiphilic block copolymer poly (styrene-b-N-isopropylacrylamide) with switchable catalytic activity immobilizing pectinase
|
|
Jiang Qin
Lei Zhongli
|
|
|
Abstract
|
|
|
|
Pectinase was immobilized onto thermo-sensitive amphiphilic block copolymers poly(styrene-b-Nisopropylacrylamide) PS-b-poly(N-isopropylacrylamide) (PNIPAM) by covalent attachment. Biochemical studies have found that the stability of the PS-b-PNIPAM support is not impeded by the bound proteins despite that up to 242.5 mg of enzyme is immobilized per gram of carrier particles. The immobilized enzyme retained nearly 65% of its initial activity over 30 days, and the optimum temperature and pH also increased to the range of 60 ∼ 70°C and 4.0 ∼ 6.0, respectively. The immobilized enzyme also exhibited great operational stability, and more than 60% residual activity was observed in the immobilized enzyme after 10 batch reactions. Moreover, the lower critical solution temperature of the PS-b-PNIPAM support could be switched on or off by a small change in solution temperature. Thus, the immobilized pectinase could be recovered and showed durable activity during the recycle process.
|
|
|
KEYWORD
|
|
|
covalent attachment, pectinase immobilization, poly(styrene-b-N-isopropylacrylamide) support, lower critical solution temperature (LCST), thermo-switchable
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|